Abstract Detail

Genetics Section

Kim, Hyi-Gyung [1], Ruck, Elizabeth [1], Guisinger, Mary [1], McMurtry, Vanity [1], Boore, Jeffrey [2], Jansen, Robert [1].

Comparative chloroplast genomics of legumes (Fabaceae).

We are sequencing the chloroplast genome of Trifolium subterraneum (Fabaceae). Previous restriction site and gene mapping comparisons indicated that this genome is highly rearranged and that it contains some novel DNA and a family of dispersed repeats. The primary goal of our sequence comparisons of Trifolium is to confirm the genome organization and to examine the role of the repeated sequences in gene order changes. A second goal of this project is to compare the Trifoliumgenome sequence with completely sequenced genomes of two other legumes, Medicago truncatula and Lotus japonicus. Differences in genome organization among these three legumes are due to insertions, deletions, inversions, and loss of inverted repeat. These comparisons will provide evolutionary insights into the mechanisms and patterns of evolution in legume chloroplast genomes. Furthermore, the availability of complete chloroplast genome sequences from three legumes will enable comparisons of rates and patterns of sequence evolution in coding and non-coding regions and between genes that are duplicated in the inverted repeat of Lotus but single copy in the Trifolium and Medicago because of the loss of one copy of the inverted repeat.

Related Links:

1 - University of Texas at Austin, Section of Integrative Biology, 1 Universiy Station, #A6700, Austin, Texas, 78712, USA
2 - DOE Joint Genome Institute, Department of Evolutionary Genomics, 2800 Mitchell Drive, Walnut Creek, California, 94598, USA

Keywords:
chloroplast genome
comparative genomics
Fabaceae
Trifolium
Medicago
Lotus.

Presentation Type: Paper
Session: 53-5
Location: Peruvian (Cliff Lodge)
Date: Wednesday, August 4th, 2004
Time: 2:00 PM
Abstract ID:854