Recent Topics Posters
Lickey, Edgar , Small, Randall , Braam, Janet , Pigliucci, Massimo .
Developmental gene sequence variability within and among natural populations of Arabidopsis thaliana.
Arabidopsis thaliana is a species that exhibits high levels of self pollination. Because of this, natural populations are presumed to be highly inbred with little intrapopulation genetic variability and high interpopulation divergence. Sequence analysis of calmodulin related genes (TCH2 and TCH3) whose expression levels are strongly upregulated during thigmomorphogenesis response is being conducted to determine the range and extent of genetic variability within and among natural European populations of A. thaliana. Analysis of TCH2 sequences indicates that, indeed, there is no variability within sampled populations, but there is considerable genetic divergence between populations. However, variability of TCH3 sequences does exist among individuals within at least three populations, and two putative heterozygous individuals have been identified. Furthermore, sequence analysis of TCH3 has revealed a puzzling phenomenon where the gene, which contains four exons and three introns, is composed of three, tandemly repeated, approximately 370 bp long segments. Several individuals in two populations have been found where the gene contains a fourth repeat, and one population was found to be composed primarily of individuals whose TCH3 gene contains only two repeats.
1 - State University of New York, Ecology and Evolution, 640 Life Sciences Building, Stony Brook, New York, 11794-5245, USA
2 - University of Tennessee, Department of Botany, 437 Hesler Biology, Knoxville, Tennessee, 37996, USA
3 - Rice University, Biochemistry and Cell Biology, MS-140, 6100 Main Street, Houston, Texas, 77251, USA
population genetic diversity
Presentation Type: Poster
Location: Special Event Center (Cliff Lodge)
Date: Tuesday, August 3rd, 2004
Time: 12:30 PM